Trypsin-ultra, Mass Spectrometry Grade (Recombinant) is a serine protease recombinantly expressed in E. coli, originating from porcine trypsin. It is engineered and further chemically modified via methylation to render it highly resistant to autolysis. It selectively cleaves peptide bonds C-terminal to lysine and arginine residues. This enzyme’s high purity and lack of contaminating enzymes, autolysis resistance, and stability make it ideal for a variety of proteomics workflows, including those using PNGase F, and applications that require regulatory compliance.
- Recombinantly produced and engineered to be autolysis resistant
- Permits rapid digest protocols
- Free of chymotrypsin
- Suitable for use in PNGase F workflows
- Accurate and reproducible, ideal for MAMs